PulseNet

PulseNet is vital to food safety. It enables the early detection, prevention and control of foodborne diseases by allowing scientists to compare the DNA fingerprinting profiles of bacteria from patients and contaminated food products. This system helps laboratorians detect and define clusters of bacterial foodborne pathogens, such as Salmonella, Listeria and E. coli O157, and identify the sources of contamination.

PulseNet utilizes whole genome sequencing (WGS)—a next generation technology that has replaced pulsed-field gel electrophoresis (PFGE) for pathogen identification—which identifies the complete genetic composition of bacteria by analyzing their DNA. Public health laboratorians use this technique to compare bacterial strains that cause illness in order to identify outbreaks. It also helps scientists understand why certain bacteria cause more severe disease or show greater resistance to antibiotics than others. APHL continues to support the implementation of next generation technologies to further strengthen the  PulseNet Network surveillance program.

APHL works with PulseNet’s federal, state and local partners to create a globally integrated surveillance network in response to foodborne disease outbreaks.

We are committed to helping PulseNet participants generate the highest quality WGS data. High quality data enhances the accuracy of outbreak detection, enabling the implementation of more timely and effective preventative measures and contributing to overall improvements to food safety.

In collaboration with the US Centers for Disease Control and Prevention (CDC), we manage the network's standard operating procedures by administering, organizing and evaluating certifications and proficiency testing.